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cd4 bead negative selection kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd4 bead negative selection kit
    A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic <t>CD4</t> + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .
    Cd4 Bead Negative Selection Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+bead+negative+selection+kit/pmc11885808-311-15-22?v=Miltenyi+Biotec
    Average 97 stars, based on 462 article reviews
    cd4 bead negative selection kit - by Bioz Stars, 2026-07
    97/100 stars

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    1) Product Images from "CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation"

    Article Title: CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-025-02371-6

    A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic CD4 + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .
    Figure Legend Snippet: A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic CD4 + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .

    Techniques Used: Knock-Out, Incubation, Labeling, In Vitro, Real-time Polymerase Chain Reaction



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    A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic <t>CD4</t> + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .
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    Image Search Results


    A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic CD4 + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .

    Journal: Cell Death Discovery

    Article Title: CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation

    doi: 10.1038/s41420-025-02371-6

    Figure Lengend Snippet: A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic CD4 + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .

    Article Snippet: Naïve CD4 + T cells were purified from the spleens of OT-II mice by a CD4 + bead negative selection kit (MACS Miltenyi Biotec) and were labeled with 5 μM CFSE (ThermoFisher Scientific) at 37 °C for 30 min.

    Techniques: Knock-Out, Incubation, Labeling, In Vitro, Real-time Polymerase Chain Reaction